Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Antibodies Used in This Study

Article Snippet: MUC16 , CUSABIO , PA10657A0Rb , Rabbit , 1:2400.

Techniques:

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Mouse TaqMan Gene Expression Assays Used in This Study

Article Snippet: MUC16 , CUSABIO , PA10657A0Rb , Rabbit , 1:2400.

Techniques: Gene Expression

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Ectoine enhances production of transmembrane mucins in DS mice. ( A, C, E , G ) Representative images of IF staining showing the decreased production of MUC1 ( A ), MUC4 ( C ), MUC16 ( E ), and MUC15 ( G ) in conjunctiva and/or cornea of DS mice (DS+PBS) was recovered by ectoine eye drops (DS+Ect); Scale bars = 50 µm. The stitched images were made because the cornea and conjunctiva in some eyeball sections were too far apart to be captured on the same image. ( B, D, F , H ) IF intensity quantified the alternation of these four mucins in the three groups; each circle or triangle represents one eye. Data are shown as Mean ± SD, n = 6; the P values are shown in the graph as compared with the DS+PBS group.

Article Snippet: MUC16 , CUSABIO , PA10657A0Rb , Rabbit , 1:2400.

Techniques: Staining, Eye Drops

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ectoine Enhances Mucin Production Via Restoring IL-13/IFN-γ Balance in a Murine Dry Eye Model

doi: 10.1167/iovs.65.6.39

Figure Lengend Snippet: Gene expression of MUC5AC, MUC2, MUC1, MUC4, MUC16 , and MUC15 by conjunctiva and cornea. ( A ) Gene expression of all 6 mucins in normal mice were detected at different mRNA levels based on the threshold cycle (Ct) value in real-time PCR with house-keeping gene GAPDH as an internal control. ( B ) The mRNA levels of all six mucins in conjunctiva of the three groups of mice. ( C ) The mRNA expression of MUC1, MUC4, and MUC16 by corneas in the three groups of mice. Data are shown as Mean ± SD, n = 6; the P values are shown in the graph as compared with the DS+PBS group.

Article Snippet: MUC16 , CUSABIO , PA10657A0Rb , Rabbit , 1:2400.

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Control, Expressing

Journal: Molecular Therapy Oncology

Article Title: Muc16CD is a novel CAR T cell target antigen for the treatment of pancreatic cancer

doi: 10.1016/j.omton.2024.200868

Figure Lengend Snippet: Muc16CD expression is upregulated in patient-derived pancreatic tumors (A) Graphical representation of Muc16 cleavage to expose the retained ectodomain (Muc16CD) on the tumor cell surface. (B) Immunofluorescence microscopy images of PDAC PDX tumors (PANC XXIII, PANC II, and PANC XXVIII). Blue is DAPI nuclear staining, and red is expression of the retained ectodomain, Muc16CD. (C) A PANCII PDX in vivo tumor was freshly harvested from mice and enzymatically digested. Live cells were analyzed for Muc16CD surface expression by flow cytometry. The negative control cell line is the mouse KPC PDAC cell line that does not express Muc16CD.

Article Snippet: To detect Muc16CD expression on tumor cells, an anti-Muc16CD antibody (clone 4H11) was generated at the Memorial Sloan Kettering Cancer Center Antibody and Bioresource Core Facility and conjugated with APC (Abcam, Cambridge, UK).

Techniques: Expressing, Derivative Assay, Immunofluorescence, Microscopy, Staining, In Vivo, Flow Cytometry, Negative Control

Journal: Molecular Therapy Oncology

Article Title: Muc16CD is a novel CAR T cell target antigen for the treatment of pancreatic cancer

doi: 10.1016/j.omton.2024.200868

Figure Lengend Snippet: CAR T cells recognize endogenous Muc16CD expression in pancreatic tumor cells (A) Endogenous Muc16CD expression on the tumor cell lines Panc1 (gray, negative control), HPAC (blue, human PDAC), HT137 (green, CAF), and PANCII PDX (orange, PDX-derived PDAC) were evaluated by flow cytometry. (B) Tumor cells were cocultured with CAR T cells at different E:T ratios. Cytotoxicity plots are representative of three independent experiments (see also Figure S1 ). HPAC and HT137 cells were co-cultured for 48 h, whereas PANCII PDX was co-cultured for 72 h. Negative control represents coculture with a Muc16CD-negative cell line (either 3T3-GFPLuc or Panc1-GFPLuc cells). Plots are representative of three independent experiments. (C and D) Muc16CD-directed CAR T cells upregulate (C) CD69 and (D) IL-2, IFNg, TNF-α, and GzmB when cocultured with PANCII PDX cells (cell line). Data represent mean with SEM in (C). The p values were determined by (C) two-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: To detect Muc16CD expression on tumor cells, an anti-Muc16CD antibody (clone 4H11) was generated at the Memorial Sloan Kettering Cancer Center Antibody and Bioresource Core Facility and conjugated with APC (Abcam, Cambridge, UK).

Techniques: Expressing, Negative Control, Derivative Assay, Flow Cytometry, Cell Culture

Journal: Molecular Therapy Oncology

Article Title: Muc16CD is a novel CAR T cell target antigen for the treatment of pancreatic cancer

doi: 10.1016/j.omton.2024.200868

Figure Lengend Snippet: Muc16CD-targeted CAR T cells improve survival in a pancreatic mouse model (A) Panc1-Muc16CD tumor cells (2.5−5E6) were subcutaneously engrafted into SCID/beige mice and subsequently treated intraperitoneally with Muc16CD-targeting CAR T cells (see also Figure S3 ) 5–10 days later. (B) Representative images of one independent experiment showing tumor burden measured by bioluminescence imaging at days 14, 28, 42, and 56. (C) Quantification of bioluminescence imaging. (D) Kaplan-Meier curve of OS (combination of three independent experiments). Data represent mean with standard deviation in (C). The p values were determined by (C) unpaired t tests and (D) log rank tests. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: To detect Muc16CD expression on tumor cells, an anti-Muc16CD antibody (clone 4H11) was generated at the Memorial Sloan Kettering Cancer Center Antibody and Bioresource Core Facility and conjugated with APC (Abcam, Cambridge, UK).

Techniques: Imaging, Standard Deviation